RNA

Part:BBa_K1777001:Design

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-13)


beta-globin intron1 SA with 3 mir-21 targeting sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed overlap region for overlap PCR. The interval sequence is specially designed to avoid higher-level structure of RNA.


Source

This part is synthesized de novo.

References